Differentiation of the Major Listeria monocytogenes Serovars by Multiplex PCR
نویسندگان
چکیده
منابع مشابه
DETERMINATION OF DOMINANT SEROVARS OF LISTERIA MONOCYTOGENES
Serovars of Lisreria l1Iollocyrogelles were determined. Sera of aborted samples (200) were collected from different hospitals in Tehran and were tested serologically by immunofluorescent antibody methods (IF tests). 137 positive sera were identified. Positive sera were tested against 12 serovars of Listeria l1Iollocytogelles separately. Titers of antibody in patients' sera for all serovars ...
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PCR serogrouping methods were used to examine strains of L. monocytogenes isolated in Japan. Among 187 strains, 99.5% were classified into 4 PCR serogroups corresponding to conventional serotypes. Only one isolate had a new PCR profile, which may be a variant of serogroup IVb.
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A classification system based on Fourier transform infrared (FTIR) spectroscopy combined with artificial neural network analysis was designed to differentiate 12 serovars of Listeria monocytogenes using a reference database of 106 well-defined strains. External validation was performed using a test set of another 166 L. monocytogenes strains. The O antigens (serogroup) of 164 strains (98.8%) co...
متن کاملdetermination of dominant serovars of listeria monocytogenes
serovars of lisreria l1iollocyrogelles were determined. sera of aborted samples (200) were collected from different hospitals in tehran and were tested serologically by immunofluorescent antibody methods (if tests). 137 positive sera were identified. positive sera were tested against 12 serovars of listeria l1iollocytogelles separately. titers of antibody in patients' sera for all serovars...
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Serotyping is a universally accepted subtyping method for Listeria monocytogenes. Identification of the strain serotype permits differentiation between important food-borne strains (1/2a, 1/2b, and 4b) and provides a "gold standard" for comparing isolates analyzed in different labs and with different techniques. Although an efficient enzyme-linked immunosorbent assay serotyping protocol was des...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2004
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.42.8.3819-3822.2004